Equipements BioCIS CY - Biochemistry

Since 2023, the BioCIS Chemical Biology team has had a Class 1 laboratory (containment class C1) fully equipped for bacterial culture, expression and purification of proteins.

purif prot
purif prot

Proteins chromatography





Cytiva äkta start in thermostated chamber (5 °C)


 

Glove box




Sylatech GloveBox in acryl (< 10 ppm)


 

GLOVEBOX
GLOVEBOX
station electrophorese
station electrophorese

Electrophoresis station

- Invitrogen Minigel Tank
- Invitrogen iBlot 2
- Invitrogen Powercase Touch 350W

- Advance Mupid-One
- FisherBrand Isotemp
- Table UV Vilber (312, 365 nm)
- Scanner

Centrifuges



- Eppendorf Centrifuge 5430R refrigerated with fixed angle rotor for falcon (7830 rpm)


- Eppendorf Centrifuge 5804R refrigerated
Rotor swing-bucket for 96-well plates, falcons (15 and 50 mL) and flasks (250 mL) (4200rpm)
Fixed rotor angle for microtubes (30 x 1.5 mL) (14000 rpm)

 
centri
centri
nanodrop
nanodrop

Nanodrop Spectrophotometer







IMPLEN Nanophotometer N50




 

Incubators





Eppendorf Thermomixer C (x3)



 

thermomix
thermomix
congelo
congelo

Freezer -80 °C






Eppendorf CryoCube 





 

Sonicator





Benchmark Pulse 150 Ultrasonic homogenizer 
with 6 mm probe for falcons and 2 mm probe for microvolumes



 

ultra son
ultra son
pHmetre
pHmetre

pH-meter




Hanna Micro with micro-electrode



 

Micro-balance







Ohaus Pioneer semi-micro (+/- 10 µg)





 

balance
balance
centri
centri

High-Speed Centrifuge



Beckman Coulter Avanti J-25 refrigerated
- Rotor JA 25.50, 8 x 50 mL, 25000 rpm, 75600 x g
- Rotor JLA 16.250, 6 x 250 mL, 16000 rpm, 38400 x g
- Rotor JLA 10.500, 6 x 500 mL, 10000 rmp, 18600 x g

 

Biophotoreactor



Multi-wavelength photoreactor with temperature control
LED max power 50W

 

biophotoreacteur
biophotoreacteur