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Equipements BioCIS CY - Biochemistry
Since 2023, the BioCIS Chemical Biology team has had a Class 1 laboratory (containment class C1) fully equipped for bacterial culture, expression and purification of proteins.
Proteins chromatography
Cytiva äkta start in thermostated chamber (5 °C)
Glove box
Sylatech GloveBox in acryl (< 10 ppm)
Electrophoresis station
- Invitrogen Minigel Tank
- Invitrogen iBlot 2
- Invitrogen Powercase Touch 350W
- Advance Mupid-One
- FisherBrand Isotemp
- Table UV Vilber (312, 365 nm)
- Scanner
Centrifuges
- Eppendorf Centrifuge 5430R refrigerated with fixed angle rotor for falcon (7830 rpm)
- Eppendorf Centrifuge 5804R refrigerated
Rotor swing-bucket for 96-well plates, falcons (15 and 50 mL) and flasks (250 mL) (4200rpm)
Fixed rotor angle for microtubes (30 x 1.5 mL) (14000 rpm)
Nanodrop Spectrophotometer
IMPLEN Nanophotometer N50
Incubators
Eppendorf Thermomixer C (x3)
Freezer -80 °C
Eppendorf CryoCube
Sonicator
Benchmark Pulse 150 Ultrasonic homogenizer with 6 mm probe for falcons and 2 mm probe for microvolumes
pH-meter
Hanna Micro with micro-electrode
Micro-balance
Ohaus Pioneer semi-micro (+/- 10 µg)
High-Speed Centrifuge
Beckman Coulter Avanti J-25 refrigerated
- Rotor JA 25.50, 8 x 50 mL, 25000 rpm, 75600 x g
- Rotor JLA 16.250, 6 x 250 mL, 16000 rpm, 38400 x g
- Rotor JLA 10.500, 6 x 500 mL, 10000 rmp, 18600 x g
Biophotoreactor
Multi-wavelength photoreactor with temperature control
LED max power 50W